Grade 12 Bio – Enzyme Lab

Enzyme Lab Purpose To comp are the exercise of the enzyme catalase, to a non-protein catalyst under contrastive conditions. Observations Observations Rate of chemical chemical re fill Interpretations A Sand - Sand piled up at the bottom of 0 - in that location is no reaction between sand and the run tube-shaped structure-shaped structure and no bubbles hydrogen peroxide, because sand does arose non terminate any catalysts or enzymes to butt in down hydrogen peroxide MnO2 - Reaction expirered right away, 5 - MnO2 acts resembling a catalyst which bubbles rose almost to the exceed of intermissions down H2O2 into weewee and oxygen the tube gas with a humiliateder activation animation B Liver - Reaction occurred right away, 4 - Liver contains mountainous amounts of the and big, blank bubbles rose the enzyme catalase, which break down H2O2. top of the scrutiny tube This made the reaction occur quick and form bubbles. Potato - in tr uth little bubbles appeared 2 - The reaction was genuinely give and from the reaction, and did not did ot occur quickly because potato rise precise postgraduate in the tube or does not have a heap of the enzyme occur quickly catalase C Used colorful + Fresh colorful - Some bubbles make and the 3 - The reaction should have had a higher(prenominal) reaction was moderate rate of reaction, because the enzymes in the utilize liver are still brisk and can be used in the reaction again. The fresh liver can alike perform this reaction with its catalase enzyme. Due to an error or impurity of equipment, the reaction was not as strong. Used liver + H2O2 - Reaction occurred quickly and a4 - The enzyme in the liver is still lot of big bubbles formed and active and can be used e very(prenominal)where again, rose to the top of the test tube because enzymes never get used up D Crushed Liver -Reaction happened immediately, 5 - Liver contains a life-size amount of and a lot of bubbling occurred catalase enzyme, which breaks down H2O2 for a while with large, white very quickly. Since the liver is bubbles, and rose to the top of crushed, this reaction occurred even the test tube faster because there is a large surface area of the liver for the H2O2 to react with. Crushed Potato - actually few bubbles that were 3 - Since the potato is crushed, there is small formed and they did not more than surface area for the H2O2 to react rise very high in the test tube with, but potato does not contain any enzymes to break down H2O2, so this reaction was moderate and did not occur quickly. E Boiled Liver No bubbles rose to the top of 1 - No reaction and bubbles formed, the test tube, and a light, white because the turn of the liver caused cover formed on top of the liver denaturing of the proteins, and with very little, small bubbles alterd catalase proteins can not function Liver at 37( C - Reaction occurred quickly and 5 - This reaction occurred and formed large, white bubbles formed and large bubbles that rose to the top elevated up the test tube because the liver is 37( C, which is normal human body temperature.Catalase ope evaluate and breaks down H2O2 trump out at this temperature. Liver at 0( C - No bubbles formed or rose to 0 - No reaction occurred when the liver the top of the test tube. No was at 0(C because this condition is reaction occurred. too cold for the catalase enzymes to function.Enzyme practise slows down at any temperature below 37( C, and it is the slowest at 0 degrees. Almost no enzyme legal action occurs. Questions 1. The differences in the rates were mainly because of the distinct temperatures of the liver, the different particle sizes of the liver and potato, and the product used with H2O2 in the reaction. The reactions that had the quickest rates had liver as the product used in the reaction with hydrogen peroxide.Also, reactions that had liver at normal body temperature (37( C), and had a big particle size for larger surface area had fast rates of reaction. 2. H2O2 breaks down when other catalysts that can be oxidise by it are present. Because H2O2 is an unstable molecule, some inorganic substances like MnO2 can be oxidized by it, and they release oxygen gas in the reaction. 3. Temperature affects the rate of enzyme action very much. At low temperatures, the enzyme activity is very slow, because the molecules have low kinetic energy and fewer collisions occur between them. This is proven in part E of the lab, where there was no reaction and enzyme activity when the liver was 0( C.At high temperatures, the enzyme activity increases because the molecules have higher kinetic energy and more collisions occur. However, the maximum temperature the enzyme get out stay active until is about 40( C. After the temperature reaches highe r than 40( C, the protein enzyme will start to denature and slow down. Particle size also affects the rate of enzyme action. Smaller particles allow the rate of enzyme activity to be very high, because there is a larger surface area for the enzyme to react with and break down. This is proven in part D of the lab, where there was a very fast and active reaction with crushed liver and hydrogen peroxide. The large surface area of the crushed liver allows more space for the enzymes to formulate on and break down hydrogen peroxide. 4.No, the results would not be different if dog liver was used for this investigation. This is because the catalase enzyme will stay active as the temperature increases, up to about 40( C. If the liver is higher than 40 degrees, the enzyme will denature and not work. Since dog liver is exactly 40( C, the results will be the same, because the enzyme will work at a temperature of 40( C, but not any higher. In conclusion, this lab helped consolidate the understa nding of how enzymes work, and how temperature and particle size affects the rate of enzyme activity. This activity was an interactive way to see how the enzymes actually function, through liver and potato. SBI 4U0-B