Total viable count

f are viable countMicrobiology practical Total and viable counts of microorganismsAbstract entranceTotal and viable counts of microorganismsThere are several(prenominal) methods for determining marrow and viable counts of microorganismsTotal Cell tally is applyViable counting are usedDetails of uses of cell counting, including their advantages and disadvantages.There are several methods for determining integral and viable counts of microorganismsInclude other methods and include references to your witnesserBrief detail of your actual experiment, mentioning the organism and which techniques will be usedTotal Viable Count This involves counting the colonies produced by viable cells down the stairs favourable growth conditions. In pour-plate method, an aliquot of suitably diluted exemplification is mixed with nutrient agar at a temperature where it is liquid. Then the diverseness is poured into petridishes and allowed to set.Alternatively an aliquot of the sample is spread ov er the agar fall out of a Petridis victimization a sterile spreader. Membrane filters can withal be used to determine the bacterial numbers. In this method cells are filtered onto membrane filter which is then placed over nutrient agar surface.Total Cell Count The most common method of enumerating the total microbial cells is the direct counting of cell suspension in a counting chamber of known volume using a microscope. sensation such counting chamber is Neubauer counting chamber. Another method involves an electronic instrument, Coulter counter.http//www.microbiologyprocedure.com/aquatic-environment-microbiology/total-cell-count.htmhttp//www.mansfield.ohio-state.edu/sabedon//biol4038.htmhttp//www.rapidmicrobiology.com/test-methods/Total-Viable-Count.phphttp//www.biochemj.org/bj/021/0104/0210104.pdfMaterials and methods1 ) A pour plate method using viable countExplain the procedure where cells crosses gridlines of the haemocytometerDiscussionIn this countersign you should disc uss the misunderstandings associated with measurement of viability. Discuss ways of improving the experiment and whether this could be achieved with the material providedThe experiment could be improved byTransferring the diluted beginning quicker to the agar plate, so that the plate will not pay contaminated by the air.The experiment could be repeated more than 3 times for a reliable testThe main source of error occurred during experiment was leaving the agar plate lid open to channelise the dilutions for a long time which could of contaminated the agar plate by air.(Madigan, 2009)ReferenceMadigan, M. C. (2009). Brock Biology of Microorganisms (12th edition ed.). San Francisco Pearson international Education.